FastQCFastQC Report
Mon 3 Jul 2017
G117C_T2_R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG117C_T2_R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences53134873
Sequences flagged as poor quality0
Sequence length50
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT74650.032.12977644
TCTCGTA68000.031.86788243
CGTCTGA96150.028.66180216
AATCTCG75150.028.57186341
GATCGGA95200.028.1806431
ACACGTC101800.027.48153713
ATCGGAA98950.026.9620482
ACGTCTG104450.026.6790215
ATCTCGT83200.026.12535742
TCGGAAG112000.024.3310413
CGGAAGA117800.022.946074
CACGTCT127950.022.12279314
CACACGT137700.020.47650112
GCACACG143750.019.58410811
GAACTCC201350.013.90523921
TCTGAAC229550.012.56108318
GTCTGAA238950.012.12217117
CCAGTCA235700.011.8507526
CAGTCAC240700.011.65026527
TCCAGTC244550.011.62873725
Produced by FastQC (version 0.11.2)