FastQCFastQC Report
Mon 3 Jul 2017
G117-M8_GTGAAA_BC72YLANXX_L007_001.R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG117-M8_GTGAAA_BC72YLANXX_L007_001.R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24510184
Sequences flagged as poor quality0
Sequence length50
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG321800.13129236402305264Illumina Single End PCR Primer 1 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGCCG41400.040.7094444
GCGTCGT44400.039.77940811
AGCGTCG44700.039.56139810
CGTCGTG45100.039.35698312
GATCGGA48900.036.7426721
TGGTCGC46150.036.37994842
GGTCGCC46450.036.14321543
GTGGTCG47800.035.6327941
ATCGGAA51650.034.7866632
GAGCGTC51650.034.6212969
GTCGTGT52650.033.5880113
TCGTGTA52850.033.29490714
GATCTCG52350.033.25091634
CGTGTAG54350.032.49741415
ATCTCGG54350.032.1086435
TCTCGGT54100.032.0129536
CGGTGGT54550.031.50681739
TCGGTGG55150.031.36355438
AGAGCGT57800.030.9755948
TCGGAAG60050.030.2884793
Produced by FastQC (version 0.11.2)