FastQCFastQC Report
Mon 3 Jul 2017
G117-M6_CCGTCC_BC72YLANXX_L007_001.R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG117-M6_CCGTCC_BC72YLANXX_L007_001.R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17918282
Sequences flagged as poor quality0
Sequence length50
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG224510.1252966104674544Illumina Single End PCR Primer 1 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGCCG26950.040.73855244
CGTCGTG29150.040.3686712
AGCGTCG29650.040.20686310
GCGTCGT29500.039.8896111
GATCGGA32450.037.2062031
TGGTCGC30250.036.00753442
GGTCGCC30750.035.4918243
ATCGGAA34350.035.2767562
GTCGTGT33550.035.271213
GAGCGTC34250.034.9352539
GTGGTCG32100.034.5510241
TCGTGTA34500.034.04541814
GATCTCG33950.033.9645434
CGTGTAG35250.033.6330415
AGAGCGT37150.032.6225858
TCGGTGG36350.031.96490138
TCGGAAG38450.031.8024373
AAGAGCG40500.030.4672647
CGGTGGT37400.030.12605339
TCTCGGT38100.030.03462836
Produced by FastQC (version 0.11.2)