FastQCFastQC Report
Mon 3 Jul 2017
G117-M6_CCGTCC_BC72YLANXX_L007_001.R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG117-M6_CCGTCC_BC72YLANXX_L007_001.R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17918282
Sequences flagged as poor quality0
Sequence length50
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTCCCGATCTCGTAT195180.10892785368597281TruSeq Adapter, Index 16 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCCCG29750.038.4441635
GATCGGA33250.037.19871
CCCGATC26050.035.12364238
CTCGTAT29200.035.02286544
GTCCCGA33300.034.87415336
ACCCGTC33200.034.846732
ATCGGAA35600.034.721842
TCTCGTA26100.034.7070643
CCGATCT26300.034.53888339
CGATCTC26200.034.23810240
TCACCCG34450.033.90152730
CGTCTGA38050.033.06399516
CACCCGT35400.032.9296131
GATCTCG28000.032.5880941
TCCCGAT31300.032.32417737
ACACGTC39250.031.77293813
ACGTCTG40600.030.82479515
CCGTCCC37250.030.7627734
TCGGAAG42000.030.0069433
CCCGTCC39600.028.77057633
Produced by FastQC (version 0.11.2)