FastQCFastQC Report
Mon 3 Jul 2017
G117-M5_ATGTCA_BC72YLANXX_L007_001.R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG117-M5_ATGTCA_BC72YLANXX_L007_001.R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25131571
Sequences flagged as poor quality0
Sequence length50
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTCGT33050.039.46447811
GTCGCCG31400.039.3798644
AGCGTCG33250.039.16086610
CGTCGTG33100.039.13898512
GATCGGA37100.035.683891
GGTCGCC34800.035.5983243
TGGTCGC34900.035.24510642
GTGGTCG36750.033.95156541
GATCTCG38650.033.4213834
GAGCGTC39300.033.300129
ATCGGAA40000.032.7674642
GTCGTGT41200.032.0318113
TCGTGTA41650.030.99988214
CGTGTAG42450.030.46778515
TCGGTGG43000.030.3479238
CGGTGGT43350.029.64602339
AGAGCGT44550.029.277128
TCTCGGT44950.028.83555436
ATCTCGG45450.028.51833335
AAGAGCG46050.028.514527
Produced by FastQC (version 0.11.2)