FastQCFastQC Report
Mon 3 Jul 2017
G117-M5_ATGTCA_BC72YLANXX_L007_001.R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG117-M5_ATGTCA_BC72YLANXX_L007_001.R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25131571
Sequences flagged as poor quality0
Sequence length50
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA37450.035.2599031
CTCGTAT34550.034.2006244
ATCGGAA39350.033.3683432
TCTCGTA33350.032.19217743
CGTCTGA43700.030.49944316
AATCTCG35500.029.68361341
TCGGAAG44950.029.4067673
ACACGTC45300.029.22799513
ACGTCTG45950.028.95813615
ATCTCGT37400.028.35245542
CGGAAGA48200.027.1500534
CACGTCT56250.024.1247814
CACACGT61250.022.26313612
GCACACG63000.021.74944911
GAACTCC95300.014.30867921
ACATGTC95750.013.80532
CCAGTCA103800.013.20053326
GTCACAT103700.012.97996229
CATGTCA102350.012.95776833
CAGTCAC107850.012.66403927
Produced by FastQC (version 0.11.2)