FastQCFastQC Report
Mon 3 Jul 2017
G117-M4_AGTCAA_BC72YLANXX_L007_001.R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG117-M4_AGTCAA_BC72YLANXX_L007_001.R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28003302
Sequences flagged as poor quality0
Sequence length50
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGCCG37850.036.27285444
CGTCGTG42250.033.78689612
TGGTCGC40950.033.2082942
GCGTCGT43500.033.01803211
AGCGTCG44100.032.518710
GTGGTCG44200.031.26618841
GATCTCG46350.030.76574934
GGTCGCC45400.030.14554643
GTCGTGT50250.028.49553513
TCGTGTA50600.028.25496314
CGTGTAG52000.027.62149415
ATCTCGG51550.027.40652535
TCGGTGG52200.026.89662638
TCTCGGT53050.026.71456336
GAGCGTC54150.026.6052289
CGGTGGT55200.025.47471839
GATCGGA60000.024.3376661
AGAGCGT60300.024.0011888
ATCGGAA61600.023.7058582
CTCGGTG59900.023.6228237
Produced by FastQC (version 0.11.2)