FastQCFastQC Report
Wed 10 Jun 2015
G117-M1_ATCACG_BC72YLANXX_L007_001.R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG117-M1_ATCACG_BC72YLANXX_L007_001.R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23371443
Sequences flagged as poor quality0
Sequence length50
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG1607370.6877495754113258Illumina Single End PCR Primer 1 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGCCG183750.042.8910744
AGCGTCG192650.042.75628310
CGTCGTG192200.042.74231712
GCGTCGT192600.042.6307111
GATCGGA199050.041.9497681
TGGTCGC187200.041.811742
GGTCGCC188450.041.71886443
GTGGTCG190750.041.5780641
GTCGTGT198800.041.46740313
ATCGGAA200950.041.3784752
TCGTGTA198650.041.30012514
GAGCGTC199900.041.18359
GATCTCG197850.041.15386234
CGTGTAG202500.040.81974415
TCGGTGG200150.040.54961438
ATCTCGG201900.040.3725435
AGAGCGT204850.040.3171848
CGGTGGT199550.040.26349639
TCGGAAG207600.040.1828773
TCTCGGT201550.040.13692536
Produced by FastQC (version 0.11.2)