FastQCFastQC Report
Mon 3 Jul 2017
G117-M15_TGACCA_BC72YLANXX_L007_001.R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG117-M15_TGACCA_BC72YLANXX_L007_001.R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29795251
Sequences flagged as poor quality0
Sequence length50
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGC3156251.059313109998637TruSeq Adapter, Index 4 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA415800.043.086291
ATCGGAA417850.042.661822
CGTATGC414450.042.26944444
CGTCTGA425250.042.1828616
TCGTATG415450.042.17343
CTCGTAT388100.042.12321542
TCGGAAG425900.042.0309753
TCTCGTA361550.041.93621441
ACGTCTG430250.041.86645515
ACACGTC431400.041.7803413
CGGAAGA427500.041.5955664
AATCTCG363750.041.35164339
ATCTCGT366750.041.23943740
CACGTCT443800.040.63279714
GCACACG447100.040.38208411
CACACGT448750.040.29731812
ACTGACC467200.037.5667132
ACCAATC398900.037.55897536
GACCAAT425200.037.18073335
GAACTCC483800.036.93691621
Produced by FastQC (version 0.11.2)