FastQCFastQC Report
Mon 3 Jul 2017
G117-M13_ACTGAT_BC72YLANXX_L007_001.R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG117-M13_ACTGAT_BC72YLANXX_L007_001.R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences33767812
Sequences flagged as poor quality0
Sequence length50
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTGATATATCTCGTAT684150.2026041841265878TruSeq Adapter, Index 25 (97% over 44bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT88500.038.158144
TCTCGTA83000.037.73872843
TATCTCG87550.035.55083541
ATCTCGT91050.034.28108642
GATCGGA130500.028.1984161
ATCGGAA135200.027.1350572
CGTCTGA142500.026.17684216
TCGGAAG145850.025.4250533
ACACGTC149200.025.20771813
ACGTCTG149700.025.13821615
CGGAAGA149500.024.7011074
CACGTCT164000.023.0133414
CACACGT171250.021.97483612
GCACACG172650.021.8985611
CTGATAT176000.020.53199435
ACTGATA177200.020.5046634
GATATAT167550.019.95287137
GTCACAC190350.019.43476729
ATATCTC164800.019.18064540
ATATATC172200.018.5966438
Produced by FastQC (version 0.11.2)