FastQCFastQC Report
Mon 3 Jul 2017
G117-M12_GAGTGG_BC72YLANXX_L007_001.R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG117-M12_GAGTGG_BC72YLANXX_L007_001.R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26616350
Sequences flagged as poor quality0
Sequence length50
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG908760.3414292342864442Illumina Single End PCR Primer 1 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGCCG103450.041.83530844
TGGTCGC107500.040.03856742
GTGGTCG111950.039.0388441
GGTCGCC111150.038.9394843
GATCTCG117500.038.2437534
CGGTGGT119450.037.38049739
TCGGTGG120600.037.3707538
ATCTCGG121550.036.98807535
TCTCGGT122800.036.53988636
CGTCGTG130900.035.13459812
CTCGGTG127600.035.09642437
AGCGTCG131000.034.88944210
GCGTCGT131700.034.85450411
GATCGGA136400.034.260791
ATCGGAA139150.033.5049362
GAGCGTC138400.033.0716449
GTCGTGT139800.033.05529813
TCGTGTA139000.032.8976714
CGTGTAG142000.032.5128715
AGAGCGT145000.031.7786758
Produced by FastQC (version 0.11.2)