FastQCFastQC Report
Wed 10 Jun 2015
G116-M8_ACTTGA_BC72YLANXX_L008_001.R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG116-M8_ACTTGA_BC72YLANXX_L008_001.R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25539172
Sequences flagged as poor quality0
Sequence length50
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG3250941.272923021936655Illumina Single End PCR Primer 1 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGTCG386800.043.2042510
GCGTCGT386550.043.1583111
GTCGCCG379050.043.11966744
CGTCGTG387300.043.1088112
GATCGGA393850.043.0037571
ATCGGAA394150.042.7601052
TGGTCGC382750.042.6079442
GAGCGTC392300.042.508839
GTCGTGT394700.042.47383513
GTGGTCG386100.042.4385641
GATCTCG393400.042.26795234
GGTCGCC386700.042.2567543
TCGTGTA393550.042.2348414
TCGGAAG401350.042.0440523
AGAGCGT398300.041.9786728
CGTGTAG397850.041.9339815
AAGAGCG398700.041.9197587
TCGGTGG401300.041.78682738
TCTCGGT398650.041.74442736
ATCTCGG398050.041.7354835
Produced by FastQC (version 0.11.2)