FastQCFastQC Report
Wed 10 Jun 2015
G116-M4_TGACCA_BC72YLANXX_L008_001.R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG116-M4_TGACCA_BC72YLANXX_L008_001.R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31533368
Sequences flagged as poor quality0
Sequence length50
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGC14965334.745871103904918TruSeq Adapter, Index 4 (100% over 50bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATATCGTATGC460350.14598821159858344TruSeq Adapter, Index 4 (98% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA1927000.043.7995151
ATCGGAA1929450.043.6101042
TCGGAAG1940650.043.4634063
CGGAAGA1936750.043.301034
CGTCTGA1947700.043.20989216
ACACGTC1955950.043.1873113
ACGTCTG1956550.043.1583315
CGTATGC1900700.043.03228844
GCACACG1967700.042.91488611
CACGTCT1972950.042.84416614
CACACGT1976150.042.8204212
TCTCGTA1716650.042.7588541
TCGTATG1913550.042.74907343
CTCGTAT1819350.042.74107742
AATCTCG1701750.042.6982639
ATCTCGT1717200.042.5641140
GAACTCC2005950.041.72489521
ACTGACC1945050.041.66198732
ACCAATC1725500.041.63767636
TCTGAAC2027500.041.51679618
Produced by FastQC (version 0.11.2)