FastQCFastQC Report
Wed 10 Jun 2015
G116-M1_ATCACG_BC72YLANXX_L008_001.R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG116-M1_ATCACG_BC72YLANXX_L008_001.R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19849169
Sequences flagged as poor quality0
Sequence length50
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATGC1155200.5819890998963231TruSeq Adapter, Index 1 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA159400.042.594461
ATCGGAA160950.042.1016542
CGTATGC158500.041.5238844
TCGTATG160600.041.0770243
TCTCGTA140300.040.97002441
CTCGTAT151600.040.88316742
CGATCTC139300.040.7343238
CGTCTGA166800.040.7299416
TCGGAAG167500.040.67813
ACACGTC167850.040.46204413
GATCTCG141600.040.33672339
ACGATCT139250.040.3067137
ACGTCTG169950.040.1820715
CACGATC139350.040.11995336
CGGAAGA169300.040.0377584
TCACGAT147500.039.7073635
CATCACG161950.039.58671633
ATCACGA161150.039.4693434
ATCTCGT145850.039.33541540
GCACACG176700.038.52263311
Produced by FastQC (version 0.11.2)