FastQCFastQC Report
Wed 10 Jun 2015
G116-M14_ACTGAT_BC72YLANXX_L008_001.R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG116-M14_ACTGAT_BC72YLANXX_L008_001.R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22578374
Sequences flagged as poor quality0
Sequence length50
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTGATATATCTCGTAT4529872.0062870780685977TruSeq Adapter, Index 25 (97% over 44bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA586200.043.293781
ATCGGAA590550.042.892082
TCGGAAG597800.042.5736773
CGTCTGA602050.042.55423716
CTCGTAT544550.042.40516744
ACGTCTG605850.042.3998615
CGGAAGA599150.042.2830164
ACACGTC605200.042.2673313
TCTCGTA514150.042.18047343
TATCTCG516150.042.0725741
ATCTCGT519500.041.76730342
GCACACG616450.041.52807611
CACGTCT619500.041.3804214
CACACGT620000.041.3364112
GAACTCC644900.039.59033221
GTCACAC626500.039.54196529
TCTGAAC655150.039.20591718
GTCTGAA659850.038.9899917
CCAGTCA650000.038.9379626
ACTGATA624650.038.8985734
Produced by FastQC (version 0.11.2)