FastQCFastQC Report
Wed 10 Jun 2015
G116-M12_CTTGTA_BC72YLANXX_L008_001.R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG116-M12_CTTGTA_BC72YLANXX_L008_001.R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22957416
Sequences flagged as poor quality0
Sequence length50
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG18692378.142192483683704Illumina Single End PCR Primer 1 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA2206900.043.820781
ATCGGAA2203900.043.7672042
GTCGCCG2094850.043.7264444
GCGTCGT2176350.043.72459811
AGCGTCG2177250.043.72331610
CGTCGTG2184100.043.70942312
TCGGAAG2210900.043.689323
GAGCGTC2176300.043.6807489
GTCGTGT2194700.043.58860813
CGGAAGA2199400.043.582144
TCGTGTA2178000.043.57296814
AAGAGCG2196600.043.5249257
AGAGCGT2195500.043.5099538
TGGTCGC2104100.043.4693742
GGTCGCC2104250.043.44963543
GTGGTCG2122900.043.41921641
CGTGTAG2196750.043.40870315
GATCTCG2153300.043.3965634
TCGGTGG2164450.043.34990738
CGGTGGT2149900.043.3249639
Produced by FastQC (version 0.11.2)