FastQCFastQC Report
Tue 21 Mar 2017
G115_M1M2.R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG115_M1M2.R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences107139390
Sequences flagged as poor quality0
Sequence length50
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT42850.09.5501442
TCGTATG44250.09.09864543
CGTATGC51600.07.97335244
TCTCGTA45950.07.804538741
GATCGGA62050.07.1376141
CGTCTGA75350.05.838897716
ACACGTC78800.05.778769513
ATCGGAA75650.05.72850752
ATCTCGT73600.05.291044740
CGATCAG46800.05.21796333
Produced by FastQC (version 0.11.2)