FastQCFastQC Report
Thu 1 Oct 2015
G114-M6_ACTTGA_AC6GPWANXX_L007_001.R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG114-M6_ACTTGA_AC6GPWANXX_L007_001.R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences25534682
Sequences flagged as poor quality0
Sequence length50
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA36050.022.5778311
TCGTGTA36350.021.36265414
CGTGTAG36300.021.2709215
GATCTCG40500.019.93871734
GTCGTGT39900.019.35170213
GTAGATC41800.019.16020431
ATCTCGG43250.018.56918735
TCTCGGT42800.018.40452236
AGAGCGT45050.018.2137328
GTAGGGA44350.018.00558718
CGTCGTG44350.018.00519812
GCGTCGT50500.015.85604611
ATCGGAA51200.015.7252252
GTGTAGG51550.015.70380516
TGTAGGG52100.015.62274717
GAGCGTC54050.015.3844179
GAGTGTA54900.014.98820627
GTGGTCG53550.014.66720141
AGCGTCG57350.014.53753310
AGTGTAG58650.014.48017928
Produced by FastQC (version 0.11.2)