FastQCFastQC Report
Tue 16 Jun 2015
G114-M4_GCCAAT_AC6GPWANXX_L007_001.R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG114-M4_GCCAAT_AC6GPWANXX_L007_001.R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24158649
Sequences flagged as poor quality0
Sequence length50
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGTAG19050.011.08569615
TAGCATA47250.010.1959399
TAATCGA18350.010.1898514
GATCGGA21700.09.73187351
CGTCGTG22600.09.53899412
TAACGCG17400.09.4819357
TCGCGAA10100.09.36687925
CGACAAT12100.09.2719211
TCGTGTA23600.09.04158714
CGACGAT15900.08.99292851
GTAGCAT57050.08.9072018
AACGCGT19000.08.6834558
TCTAACG18900.08.61300855
ATCGCGA10450.08.42145324
AATCGAA30000.08.3592735
GTCGTGT26300.08.28064913
GATCTCG26550.08.20444334
TCTCGGT26650.08.17365636
GTAGGGA31900.08.06858218
CGAACGC16100.08.0614121
Produced by FastQC (version 0.11.2)