FastQCFastQC Report
Tue 16 Jun 2015
G114-M2_TGACCA_AC6GPWANXX_L007_001.R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG114-M2_TGACCA_AC6GPWANXX_L007_001.R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21797760
Sequences flagged as poor quality0
Sequence length50
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTCGT13750.012.15906211
CGTCGTG15400.011.71338212
CGTGTAG17300.09.28254215
TCGTGTA18850.09.10274614
GATCGGA19750.08.6879581
TCGATCG7900.08.3537867
GTCGACA24150.08.2892341
AGCGTCG21300.08.055716510
CGACGAT13350.07.90952061
ATCTCGG24950.07.583990635
TCTCGGT25700.07.448280336
GATCTCG23250.07.38144734
GTAGGGA28200.06.943029418
CGACCGT12250.06.82455821
GGAAAAT111350.06.77632241
GTCGTGT27950.06.76870813
GAGCGTC27100.06.73748739
AATCCGC14200.06.66147335
TGGTCGC30700.06.59249142
GTCGCCG27500.06.559509344
Produced by FastQC (version 0.11.2)