FastQCFastQC Report
Sat 19 Mar 2016
G113-M6_CGATGTAT_AC67REANXX_L002_001.R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG113-M6_CGATGTAT_AC67REANXX_L002_001.R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences250000
Sequences flagged as poor quality0
Sequence length50
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGC35411.4163999999999999TruSeq Adapter, Index 2 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACACGTC4100.043.41441713
GATCGGA4250.042.6980971
TCGGAAG4250.042.399213
CGTCTGA4300.041.99017716
CGGAAGA4300.041.9061974
CACACGT4300.041.90619712
ACCGATG4200.041.865932
CGATGTA4200.041.865934
CTCGTAT4100.041.80647742
TCTCGTA4050.041.7800141
ATCGGAA4350.041.4245152
CACGTCT4300.041.40342714
GTCACCG4200.041.38396529
TCACCGA4200.041.35912330
CACCGAT4200.041.35084531
TCGTATG4200.041.33430543
TATCTCG4100.041.27049639
ACGTCTG4350.040.952115
CCGATGT4300.040.89227333
ATCTCGT4150.040.77326240
Produced by FastQC (version 0.11.2)