FastQCFastQC Report
Sat 19 Mar 2016
G110_M3.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG110_M3.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences250000
Sequences flagged as poor quality0
Sequence length50
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG207.850976E-443.992843
CTCGTAT250.00235112335.1942442
CATCTCG353.2137052E-431.42342839
GATCGGA300.00573539129.33441
ATCTCGT300.00574100229.32853540
CGTATGC300.00574100229.32853544
ACTTAGG450.001394821424.44533332
CACACGT500.002568247522.00960512
GGGAGTT953.3321223E-416.2111171
GCTCTGT850.002970384415.5299775
GGCAGCT2403.521017E-510.08371
Produced by FastQC (version 0.11.2)