FastQCFastQC Report
Wed 19 Jul 2017
G110_M7.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG110_M7.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10929887
Sequences flagged as poor quality0
Sequence length50
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGC318390.29130218821109494TruSeq Adapter, Index 7 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA38300.040.2001841
CTCGTAT39950.038.3759142
CGTCTGA42600.036.7588116
ATCGGAA42250.036.1815262
ACGTCTG44050.035.39935315
CGTATGC44100.034.9119744
ATCTCGT44150.034.83169640
TCGTATG48750.031.40301543
GCACACG50700.030.84286111
CATCTCG50750.030.56727639
CGGAAGA52700.030.0085544
ACACGTC55800.028.10336113
CACACGT59500.026.35551612
ACCAGAT60550.025.92612332
TCTCGTA60050.025.71547741
GATCATC61750.025.0637936
TCGGAAG63500.024.3860593
CACGTCT67050.023.45363814
AGAGCAC68200.023.4118298
GTCTGAA68600.023.4025917
Produced by FastQC (version 0.11.2)