FastQCFastQC Report
Wed 19 Jul 2017
G110_M3.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG110_M3.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12012103
Sequences flagged as poor quality0
Sequence length50
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT9200.020.56183842
GATCGGA9200.020.3224091
TCGTATG9350.019.99621243
CGTATGC9550.019.34647844
ATCTCGT13800.014.82568740
ATCGGAA12650.014.6062342
CGCGTAT1100.001020324613.9974671
TCTCGTA13700.013.809141
ACACGTC14800.013.52412813
CGTCTGA15650.012.92981316
ACGTCTG16650.012.41775915
CGCCCAT12800.011.513541
CGGGGAT13250.011.454531
CATCTCG18450.011.32954139
CGCCTAT4153.6379788E-1211.1305151
GTCGGTT9350.011.0568231
CGGCGAT3451.9172148E-910.8386171
CGGAAGA21700.010.2380334
TCGGAAG20250.010.1022543
CACGTCT20700.010.09445514
Produced by FastQC (version 0.11.2)