FastQCFastQC Report
Wed 11 Mar 2015
G104_M3.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG104_M3.fastq
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences8316309
Sequences flagged as poor quality0
Sequence length45
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCG117510414.130114693910484TruSeq Adapter, Index 9 (100% over 45bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA1300050.038.8405571
ATCGGAA1302150.038.7769282
GATCTCG1248150.038.71640439
ACACGTC1297750.038.66825513
CGTCTGA1297350.038.6481516
TCGGAAG1306800.038.631493
CGGAAGA1307600.038.624264
ACGTCTG1297750.038.61971715
GTCACGA1252250.038.6041629
ACGATCA1246000.038.5975232
CGATCAG1243900.038.59371633
TCACGAT1247800.038.568430
AGTCACG1255250.038.5631528
GCACACG1304800.038.5534611
CACGATC1248900.038.54379731
CACGTCT1300950.038.5416714
CACACGT1301450.038.5343512
GATCAGA1255400.038.1955934
GAACTCC1315300.038.19437421
ATCAGAT1257750.038.1180235
Produced by FastQC (version 0.11.2)