FastQCFastQC Report
Wed 11 Mar 2015
G104C_M3.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG104C_M3.fastq
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences19182416
Sequences flagged as poor quality0
Sequence length40-45
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCG11751046.125943676750624TruSeq Adapter, Index 9 (100% over 45bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCTCG1254050.082.43500539
TCAGATC1286750.079.7943736
AGATCTC1299500.079.6283238
ATCAGAT1288150.079.6201535
CAGATCT1304450.078.8780837
GATCGGA3404350.036.0395431
ATCGGAA3410100.035.983062
TCGGAAG3416500.035.8997733
CGTCTGA3405250.035.8919316
ACACGTC3407800.035.88913713
CGGAAGA3418500.035.873484
ACGTCTG3406800.035.86657715
CACGTCT3413550.035.80696514
GCACACG3419450.035.80652211
ACGATCA3307950.035.80013332
GTCACGA3314650.035.79704329
CGATCAG3313750.035.79640233
CACACGT3416550.035.79351812
AGTCACG3319500.035.78069328
TCACGAT3309300.035.7806130
Produced by FastQC (version 0.11.2)