FastQCFastQC Report
Wed 11 Mar 2015
G104C_M2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG104C_M2.fastq
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences22377493
Sequences flagged as poor quality0
Sequence length40-45
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCG10104294.515380699705727TruSeq Adapter, Index 8 (100% over 45bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AATCTCG1068200.081.082439
TTGAATC1102000.078.2457636
GAATCTC1111900.077.7448238
TGAATCT1116550.077.2445137
CTTGAAT1129600.076.3739435
GATCGGA2950650.036.0800971
ATCGGAA2955400.036.023782
ACACGTC2955600.035.89407713
TCGGAAG2967250.035.8793073
CGTCTGA2954750.035.8754516
ACGTCTG2955300.035.86326215
CGGAAGA2970100.035.834524
CACGTCT2961150.035.8090814
GCACACG2967950.035.7983911
CACACGT2963600.035.78924612
GAACTCC2993050.035.41892221
GTCTGAA3000300.035.34502417
ACTTGAA2859800.035.3254934
AGAGCAC3016600.035.2826968
CACTTGA2877600.035.26584633
Produced by FastQC (version 0.11.2)