FastQCFastQC Report
Fri 2 Jun 2017
G102C_M4_40bp.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG102C_M4_40bp.fastq
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences24475025
Sequences flagged as poor quality0
Sequence length40
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAA458030.18714179045782384TruSeq Adapter, Index 12 (100% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA56050.029.8556861
ATCGGAA57700.028.904942
CGTCTGA57900.028.68401116
ACACGTC60700.027.5011113
ACGTCTG62100.026.77140415
TCGGAAG65000.025.789473
CACGTCT68850.024.41854514
CGGAAGA68700.024.3262774
CACACGT74000.022.69617512
GCACACG76350.021.86410111
CCTTGTA92850.017.94162833
GAACTCC103050.016.64605921
CTTGTAA100850.016.54403734
AGTCACC103050.016.36369328
ACCTTGT103150.016.18303332
CACCTTG105900.015.82700131
GTCACCT106550.015.71449629
TCTGAAC109800.015.56026118
TCCAGTC110650.015.34807625
CCAGTCA111350.015.2206226
Produced by FastQC (version 0.11.2)