FastQCFastQC Report
Fri 2 Jun 2017
G102C_M3_40bp.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG102C_M3_40bp.fastq
File typeConventional base calls
EncodingIllumina 1.5
Total Sequences32626059
Sequences flagged as poor quality0
Sequence length40
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA28200.020.8063091
ATCGGAA31700.018.5567862
CGTCTGA32700.017.830716
ACACGTC32700.017.51890213
ACGTCTG37150.015.83212815
TCGGAAG41700.014.4328613
CACGTCT43250.013.44202414
CGGAAGA47950.012.5870814
CACACGT52200.011.36526612
GCACACG57850.010.49035511
ACTGACC83950.06.844042332
GAACTCC93800.06.760392721
TCTGAAC101000.06.27835818
TGACCAA95500.06.144295734
GTCTGAA107550.06.006394417
TCCAGTC106850.05.982049525
CCAGTCA109650.05.829194526
CACTGAC112400.05.625917431
CAGTCAC110400.05.62018327
AGTCACT113300.05.50621128
Produced by FastQC (version 0.11.2)