FastQCFastQC Report
Fri 9 Jun 2017
SRR1178069_R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1178069_R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27722265
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACAAT98900.014.3454161
CTAGCCT117050.011.9588331
CGCCGAT18800.010.8529761
ACTAGCC94750.010.40940955
CGGGAAT40200.010.2690771
CGGGCAT62400.010.189631
CCACTAG102550.010.1268043
GATCGTA36150.010.1000715
GCGGAAT65150.010.0508531
CGACGAT25600.09.8236781
CTCGGAT88050.09.4846541
CTTAGAT84800.09.2886051
CCGGCAT61600.09.2435481
GTCGAAC21350.09.112221
TAGCCTC143100.09.0134952
CGGCAAT32200.08.9890171
CGCGGTT24500.08.909021
GGGATCG67600.08.9088363
CAATAGA176600.08.8879434
CCGGGAT102650.08.8752471
Produced by FastQC (version 0.11.2)