FastQCFastQC Report
Fri 9 Jun 2017
SRR1178066_R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1178066_R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences56193870
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGC10094811.7964254819965237TruSeq Adapter, Index 4 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGAA2058200.059.753572
GATCGGA2072550.059.50681
CGGAAGA2082600.058.9574664
TCGGAAG2132000.057.5780373
GAGCACA2445900.050.283889
AGAGCAC2465550.049.9924968
AAGAGCA2606700.047.1908457
GAAGAGC2619850.047.029986
GGAAGAG2910050.042.620195
CGTATGC1647400.035.98826644-45
TATGCCG1685850.035.2640246-47
TGCCGTC1727400.034.66764548-49
AATCTCG1634000.034.29789738-39
ATCTCGT1644600.034.2967240-41
GACCAAT1767850.033.59823634-35
CTCGTAT1702800.033.26533542-43
TCTCGTA1650550.032.952140-41
TCGTATG1750950.032.87009442-43
CACACGT1952700.032.6513912-13
CACGTCT1956350.032.4935314-15
Produced by FastQC (version 0.11.2)