FastQCFastQC Report
Mon 5 Jun 2017
SRR1178060_R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1178060_R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15903449
Sequences flagged as poor quality0
Sequence length100
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGTAT4602.3788743E-814.1760091
AGCGTCG123350.012.18615994
GTCGAAC17350.012.1427841
CGTCGTG87900.011.61167394
CGCGAAT13000.011.5756751
GATCGTA18500.011.4979055
GCGTCGT95700.011.15005294
CCGGCAT30600.011.1469471
CGCGAGT6351.3173485E-711.05917551
GATCGGA409850.011.04805694
CGACAAT28300.010.6348961
ACTAGCC47500.010.5846745
CCACTAG55700.010.1276413
AGATCGG506650.010.11847994
TCGGAAG362400.09.89583194
GAGCGTC161300.09.894299594
CGAATCG6956.1985884E-79.8811823
ATCGGAA371800.09.75794494
CCGCGAT11401.0913936E-119.6802431
CGGGTAT17150.09.6520211
Produced by FastQC (version 0.11.2)