FastQCFastQC Report
Fri 9 Jun 2017
SRR1178044_R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1178044_R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17626121
Sequences flagged as poor quality0
Sequence length100
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGAAC17850.014.7505231
GATCGTA20900.013.7275935
ACTAGCC52850.012.3703135
CGGCGAT14050.011.7124921
AGCGTCG204500.011.45833294
CGGGAAT30400.011.4449981
CCACTAG62700.011.2493093
CTCGTAT46950.010.8154781
GGATCGT31250.010.6860994
CGACAAT44900.010.5762721
CTAGCCT73200.010.5339281
CGTACTA24750.010.4519758
CGTCGTG147900.010.26647994
GCGTCGT165000.010.25340194
CGGGAAC51800.010.16591
CGAATAT42200.010.1387991
ATCGGAA569100.010.07946694
CGCGTAT8007.3196134E-99.9911741
GATCGGA621350.09.98611194
AAGAGCG324850.09.91102494
Produced by FastQC (version 0.11.2)