FastQCFastQC Report
Fri 9 Jun 2017
SRR1178002_R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1178002_R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18844950
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTAGCC67450.020.0719915
CCACTAG79200.017.6399023
CACTAGC88600.015.602984
CTAGCCT90150.015.0697466
CGACAAT49050.014.0455391
CGCGTAT4856.561095E-913.7149981
CCCCACT143450.013.7122651
TAGCCTC108150.012.428197
GTCGAAC15000.012.0365431
CGCAGAT31650.010.8085691
CTTAGAT59350.010.8074311
CGGGAAT29150.010.5945911
CTCGTAT54000.010.2064251
CGCGGTT18900.010.0555911
GATCGTA28050.09.9918685
CGGGTAT24850.09.9422881
CGCGATA4801.8196201E-49.9010752
CGCGAAT16000.09.7994881
CGGGCAT43450.09.6228181
CGGCGAT16750.09.3607041
Produced by FastQC (version 0.11.2)