FastQCFastQC Report
Fri 9 Jun 2017
SRR1177979_R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1177979_R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17620163
Sequences flagged as poor quality0
Sequence length101
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACAAT48900.015.055241
GTCGAAC17800.013.3418141
CTAGCCT72450.011.4726711
CGTATGC49300.011.22520844-45
GATCGTA20400.010.9438665
CGGGAAT27850.010.5738071
CCGGCAT36750.010.2102091
GGATCGT29600.09.9485244
CGACGAT18150.09.6825551
CTCGAAC35850.09.6716051
TAGCCTC82200.09.6496052
CCGCGAT12450.09.5375211
CGGGCAT43450.09.5103031
TATGCCG59600.09.40431346-47
ATCACGA61000.09.38238934-35
ACTAGCC51350.09.3429495
CGGCAAT27250.09.0636211
CGCGATT5254.5002886E-49.047021
GGGATCG41550.08.916253
CGGGGTT33850.08.8398881
Produced by FastQC (version 0.11.2)