FastQCFastQC Report
Fri 9 Jun 2017
SRR1177978_R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1177978_R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19578080
Sequences flagged as poor quality0
Sequence length101
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTAGCC68500.013.1685815
CCACTAG75800.013.0858853
CGACAAT49300.013.0858391
GTCGAAC19650.012.3116771
CTAGCCT91050.010.5240051
CCCCACT139350.010.4505941
GATCGTA23200.010.4365785
CACTAGC94450.010.1520624
CGCCGAT14950.09.8362621
CGCGAGT10851.2732926E-119.6183941
CTGAAAT189500.09.5122661
AATCGTG26200.09.4227575
CTCGATT36100.09.3295521
CGGAAAC72000.09.22371
CCGAAAT41550.09.1333181
CCGGGAT61500.09.1015741
CCGGAAT34100.09.0420851
CTGGAAT208200.08.9540891
TAGCCTC99750.08.90041357
CGGGAAT34450.08.8125251
Produced by FastQC (version 0.11.2)