FastQCFastQC Report
Fri 9 Jun 2017
SRR1177978_R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1177978_R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19578080
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGTA24250.012.9269025
GTCGAAC19150.012.649121
ACTAGCC60000.012.4282695
CTAGCCT83350.012.3085551
CCACTAG72900.010.4244073
CGACAAT52300.010.2620871
GGATCGT35950.010.0409224
CGTATGC55250.09.71415644-45
GGGATCG49000.09.6931043
TAGCCTC101250.09.6165162
CGCGGAA12450.09.5373911
CGGGAAT29550.09.4831681
CACGAAT92700.09.3250381
CGCCGGT16950.09.2470481
GCGGAAT38100.09.2249851
CGTATGA51800.09.077463
CTCCGTT36200.09.05314351
CTGAAAT192850.09.0386881
CGGGCAT48450.09.0188871
CGGGTAT28800.08.9055391
Produced by FastQC (version 0.11.2)