FastQCFastQC Report
Fri 9 Jun 2017
SRR1177964_R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1177964_R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19342910
Sequences flagged as poor quality0
Sequence length101
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCACTAG73400.016.8166373
ACTAGCC72100.015.6737215
CTAGCCT94650.013.4329541
AATCGTG45450.013.2678415
GATCGTA28100.013.18015
CACTAGC92250.012.7642224
ATCGTGC50700.012.2685976
CCCCACT156300.011.382291
TAGCCTC105250.011.1815952
CGTGCAT50750.011.1338048
AAATCGT58050.011.12361054
CTCGATT35150.010.6625021
CGCGAGT11000.010.3508471
CTCGTAT55650.010.2299481
GAAATCG73100.010.1963373
TCGTGCA58400.010.0818817
ATCGTAC36450.09.9002836
TCCGCGA18750.09.876347
CGATTAG26700.09.7804874
CGCGTTT10709.094947E-129.7543041
Produced by FastQC (version 0.11.2)