FastQCFastQC Report
Fri 9 Jun 2017
SRR1177963_R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1177963_R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17897455
Sequences flagged as poor quality0
Sequence length101
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AATCGTG39000.013.3968785
CGCGTAT3751.4812747E-512.666141
GATCGTA26500.012.3674115
ACTAGCC62150.011.9986955
CCACTAG67300.011.9980153
AAATCGT48800.011.2905144
ATCGTGC43150.011.2279946
TCCGCGA17450.011.1612427
CTAGCCT85100.010.995431
CGACAAT29600.010.9117081
GAAATCG60550.010.197763
ATCGTAC31850.010.1410366
CCGCGAT16850.09.8674648
CGTGCAT47500.09.7009798
CGAAAAT44100.09.585771
CCCCACT141300.09.4794361
CTCGTCT46600.09.47921851
CGCAAAT20550.09.4764921
CGCGATG19450.09.2811429
CACTAGC84300.09.2404224
Produced by FastQC (version 0.11.2)