FastQCFastQC Report
Sun 6 Aug 2017
CEBPb_Veh_Reps_Combined.R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameCEBPb_Veh_Reps_Combined.R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences82125344
Sequences flagged as poor quality0
Sequence length50
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA23600.010.06883051
CGTATGC27650.08.83171244
CTCGTAT26550.08.78342142
TCGTATG30450.07.87510243
TCTCGTA26800.07.79868141
ATCGGAA31700.07.634772
CGTCTGA36850.06.925135616
ACGTCTG45500.05.89866315
TCGGAAG48850.05.62966543
ATCTCGT45200.05.305441440
Produced by FastQC (version 0.11.2)