FastQCFastQC Report
Sun 6 Aug 2017
G154-M6-Index6_GCCAAT-_ACB6A7ANXX_L007_001.R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG154-M6-Index6_GCCAAT-_ACB6A7ANXX_L007_001.R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20030299
Sequences flagged as poor quality0
Sequence length50
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGTCG2900.015.93098710
CGTCGTG3400.013.58778712
GCGTCGT3700.012.48629411
GATCGGA4601.8189894E-1210.5297581
GTCGCCG3502.9946314E-68.80063444
GTCGTGT7551.6370905E-117.575890513
GAGCGTC6704.5874913E-97.22389559
TCGTGTA8703.0504452E-96.32161514
TGGTCGC4902.5775854E-46.28615142
GTGGTCG5653.7799175E-56.230381541
CGTGTAG9251.8299033E-96.183394415
ATCGGAA7951.344888E-76.09287362
TCGGTGG9052.847064E-75.59080938
AGAGCGT10755.4929842E-85.3212078
Produced by FastQC (version 0.11.2)