FastQCFastQC Report
Sun 6 Aug 2017
CEBPa_TCPO_Reps_Combined.R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameCEBPa_TCPO_Reps_Combined.R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences79996656
Sequences flagged as poor quality0
Sequence length50
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA22900.011.33750251
CTCGTAT24000.010.633635542
CGTATGC25450.010.37337544
TCGTATG27900.09.69901343
TCTCGTA27600.09.00763741
CGTCTGA35250.07.98837416
ATCGGAA31950.07.91935732
ACGTCTG45200.06.71653915
ATCTCGT44300.06.009279340
TCGGAAG49250.05.71793273
ACACGTC49250.05.67278613
CGGAAGA58050.05.2298944
Produced by FastQC (version 0.11.2)