FastQCFastQC Report
Wed 22 Aug 2018
ChIPseq-CAR-TCP-rep-3.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameChIPseq-CAR-TCP-rep-3.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11155689
Sequences flagged as poor quality0
Sequence length50
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT273950.2455697716205606No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGATCT37400.035.936759
TCCGATC38100.035.2764938
CGATCTA12700.033.429410
TTCCGAT40300.033.241567
ACGCTCT40300.032.195121
CGCTCTT40750.031.9022462
CTTCCGA45700.029.5543466
TCTTCCG46100.029.2501935
ATCGGAA16050.022.61484744
CGATCTC17750.022.30741110
CGATCTT16100.021.17780110
GATCGGA23450.016.32266844
GCTCTTC85300.016.2462433
CGATCTG12300.015.55926810
CTCTTCC115200.012.4496334
AGATCGG34700.011.91826844
TAGATCG13400.09.52155644
CACGACG5752.1827873E-118.79887714
ACGACGC5853.274181E-118.64846915
CGACGCT6657.2759576E-128.26962116
Produced by FastQC (version 0.11.2)