FastQCFastQC Report
Wed 22 Aug 2018
ChIPseq-CAR-CTL-rep-4.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameChIPseq-CAR-CTL-rep-4.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13010807
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTCAGAATCTCGTAT2410541.8527213569458068TruSeq Adapter, Index 15 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTCTT330600.040.04885552-53
GCCGTCT331650.039.7574250-51
CGTCTTC331200.039.73975852-53
TGCCGTC337550.039.37196750-51
ATGCCGT336100.039.3652448-49
CGTATGC350400.038.27364346-47
TATGCCG352600.037.95404448-49
TCGTATG354800.037.77892344-45
CTCGTAT354650.037.73465744-45
TCTCGTA357100.037.26302342-43
GCTTGAA333400.036.78587760-61
CGGAAGA753150.036.3635374
ATCTCGT369300.036.2120242-43
AATCTCG370500.035.9729940-41
GTATGCC371250.035.95786746-47
TCGGAAG765600.035.840423
GAGCACA762300.035.7028279
ATCGGAA771950.035.5579032
TGCTTGA356450.035.0598160-61
GATCGGA788900.034.6147581
Produced by FastQC (version 0.11.2)