FastQCFastQC Report
Thu 4 Jan 2024
G219M19_CKDL230043734-1A_22GGGVLT3_L8_R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG219M19_CKDL230043734-1A_22GGGVLT3_L8_R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20306855
Sequences flagged as poor quality0
Sequence length150
%GC53

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG786310.38721407130744767No Hit
AGACTAAGTGGCTGCTACTCGCTTGTCCAGCACAGATAGCTTCTTTCCCA624780.30766950372177276No Hit
ATAGCTTCTTTCCCACCACCTGTCGTTCAGGAAGCCTGGTGCAGGCGGCA459540.22629796686882336No Hit
ATGGAAAAGCTGCCAGAACAAAGGGGTCAGCTCCAGCAGCAGGCACAGGC425460.2095154567263124No Hit
GGCAGGCGGGCGGGCGGGCAGTGTCCCTGAATTACTCAACAGAGCCATCT384540.18936462588618475No Hit
TGAGAGTCAGCACACAGAGGCTATGGTCCAGGGGTGACCACGGTTCACTT370920.18265753116373756No Hit
TACGCCACAAAACACAGGGCAACAGATGCTCCGCAAATGCCCGTGTAGGA366840.18064835741428203No Hit
CTGGTGCAGGCGGCAGCCCAGAGCAGCATGTCAATAGCCAGGCCAGACAC364760.17962407275769685No Hit
AGGGTTTGAGAACTCCATTTCCCAGAGTGCTGTGCGGGTCGAGGGTGGTG350760.1727298491076043No Hit
GGGACAGGAGCTAAAGTCCAACTCTGTCAGCAAGCTGCTAGCCAGATGGG328450.1617434112766354No Hit
GGTCAGATAGAGACAGTATACACTCTTGTGACCCCATGAGAGAGGACCAA325110.16009864649154187No Hit
AAACTATAAGGACCCACCAGCTGATAAAATATAAATTATTCACCAACACA317670.15643485906606414No Hit
GACACAGGGCATCAAAAGGGACTGCCCAGACCAAAGTCAACAGTACTAGG309420.1523721915579739No Hit
GGCAGGAGCAAGGTCCTGGTGTCAGGCAAGTTGATGGTGGCACTGTGCCA289710.14266610954773645No Hit
ACTTCATGGCTCCCACTGGGAATCCTGGGCTGGAATACTGACCCAAGTTC283770.13974098894191148No Hit
TTATTCACCAACACACAGATCAGTAGGCAGACAGTTGTAGGAAGTCACCC279320.13754961071027494No Hit
AAGGGCAAAGGGCAGGAGGAGGGCACCAGCTGGTTACTGCGGAGATAAGA266840.13140390277076386No Hit
GAGCGAGTGAGCCACCTGTGGCGCCAGCAGGGTCTGAGCTGGCTCACCTC255150.1256472260229366No Hit
AATGCCCGTGTAGGAGTGCGACTCACCCCTTACATGGCAGGCGGGCGGGC255070.12560783045922178No Hit
AAGGACAGGTAAAGTCCAAAGTGAGGGAGTGAGTCACTGCCTTCACAGGC254260.12520895037660928No Hit
TCCGACTTTCATCCCTGGCTCTGTGAGATAAGGCCTAACAGCAAGGCCAT238050.11722642427889499No Hit
GCTCAGGAGAGAGAGGGCGCTGTTTCTTGGTGGCTCTGGGTGCAGAGGCC237480.11694573088742694No Hit
AACCTTGAAAGCTGCAAAAGAGAAACAGCACGTCCCAACAAGGGAGGCAT237210.11681277085988943No Hit
CAGGCTGGGGCCCTTGTGGCAGCCAGGTGTGGCTGATGGAAAAGCTGCCA233600.11503504604725842No Hit
GAGATAGGGAAGGATCCTATAAACTCAAAGGAAAATTCTGCCAAGAGGAT228960.11275010335179919No Hit
CGGCTGTGCCCCAGAGCCTGGGCTGTTGGTTGCCATCCAGGGCAGAGGCC228540.11254327664229641No Hit
AGGAGTCACGGATTTACAAAGCTATGTCATGACACATTCAAGCTGCCCAG225550.11107086744845522No Hit
GCAGCCACCATCCAGACAAGGCATCGCCATAGTTGGTGAGGTCTGTCTCG216490.10660931985775246No Hit
TACTGACCCAAGTTCAGTGCCAGATCAACCAGCTTGGCAGGAGCAAGGTC213700.10523539957319832No Hit
GGCTCAGCCAGTCAGCCAGGTACAAGGTCGGTCGCCCTGACTTGCCGCCA213540.10515660844576868No Hit
GATCACTGAGGTGACATCTTCTGGGAAGGGTTTCCTGAGAGTCAGCACAC211570.10418649268929138No Hit
CCAGTAACCACAGGCTAGATTGACAAAGATATAGGGAGAGAAGTAGAGAA208740.10279287462287981No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)