FastQCFastQC Report
Thu 4 Jan 2024
G219M19_CKDL230043734-1A_22GGGVLT3_L8_R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG219M19_CKDL230043734-1A_22GGGVLT3_L8_R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20306855
Sequences flagged as poor quality0
Sequence length150
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCATGGAGAGTCCAAGAGCCAAACCCGGAGGTGAGCAGAGTCCAAGAGC635300.3128500203502709No Hit
AGGCTGGACCGGCAGTTGATGGCAGAGATTCAGGCATCATGGAGAGTCCA434430.21393268430783594No Hit
CCTCATCCTGGATGCTGCTCTTGCCATGGAGTTGCTGTGTACCTCTTTGC397260.19562852051684027No Hit
CCACCCTGTCCCAACCCCCATGCCCTGTTCCTGCAGAGCCTGGCTCAAAG378600.18643950528035977No Hit
GCTTCCCAGTCTGTGTTCTCCCACTTGGGGGGTAGAGGAACGGCAGACTT378480.18638041193478755No Hit
CCGGTCAGGTTCAGGCATTGACTACACAAGTACCCAGGCTGGACCGGCAG367040.18074684632356908No Hit
GCTGCCTCTGCCGCCGCCTCCCCTGGGCTCCGGCCTCTTCCAGGCCGCGC363410.17895927262000935No Hit
GGCTGGATGCTGCTTGTTGACTTGCTCCACATGAGCAGTGCCAAGCCTCA343970.1693861506373094No Hit
CCTTGAGGTCAAGGACTTTAGCCTGAGTCTGAGTTGGAGTTTTGTCAACC327280.16116725115730623No Hit
CCACTCTTCCCTTCCCTTTGGGGCCTGAAGCCTTCTTGGTCTAATCTGTT308530.1519339159116466No Hit
TCCCATCATGTTCCTCAGGGCTAGAATGAGGATCTGAGCCGACCAGTTAA300380.14792049285819986No Hit
TCCCCTGAGATGTGATCCTGAGACAGAATCCCAGGGCAAAGTCCTGCAAG300030.14774813726694755No Hit
TAGGAGGAAGGTCAGAAAAACATGGTGATTTCGGGCACAGAATCTGTACT280860.1383079753117851No Hit
GGCTGGGAAAGTCTGAAACTGCACCCAGATGGCCTTGCTGTTAGGCCTTA280510.13813561972053282No Hit
GAGTACCACTTTTGTGCGATTCTGCATACTTATGGCCTTGAGGTCAAGGA272510.13419606334905135No Hit
CCTTTTGTGATGTTCAGCTAATGCCTGTCTGGATCAGGACACAATCTTGA265830.13090653377886433No Hit
CCAGGTCTGGGGGAGTTGGGTCTTGCTCTGCTGCAGTTTGGCTGTGACCC252920.12454907468438614No Hit
CACGCCACGCTGGAGCAATGGAGCAGGTCTTCCCCTAATGAGCTGCACAC250990.12359865670976623No Hit
GAAGTCATGGTTGTTTGGATAGTTCAGGCCAAACAGGGCCATTTAGTTGG244450.12037806937608014No Hit
AATGCCCTTCACCCCTGCAGCCCAGACAGGTCTTGGACCTTGTGCTTTGC244170.1202401849030783No Hit
AGGAACGGCAGACTTCCTTTTCATGGTGGTGAGTGCCACCCTGTCCCAAC237000.11670935750513803No Hit
TGGATTTCTGCTGAAGTCTCCTGCTGGAACGAGAACAGCACTATTGCGTT232810.11464601485557463No Hit
TGTGTACCTCTTTGCCTCTAAAGGACATCAGCAGGGCCAGCTTGTGCTGT230030.11327701901648482No Hit
CCTTTCCTCCTGACCCTGCTGTTTAATTTGGCTACTGTCCGAGGCAGAGG227600.11208037876864735No Hit
TTCAAACACAGTAAGCCTGCAAAATATTTGACAAAGGCCAGTTCATGCTC227540.11205083209586122No Hit
GCTAACATGGCTTTCTGTAATTGAATGCAATTGTATTTTCTATGCTTGTC217330.10702297327675801No Hit
TCTGACCTCTGCGTGAGTCAACCCACAGACAATGACCTCAGAGGCACCAA209810.10331979028756545No Hit
AGGACACAATCTTGAAGAGGTGCACTCCAGTGACTTAGGAGGAAGGTCAG207160.10201481223951223No Hit
CAGTGCCAAGCCTCAGTTGCCCTCCAAGACCTTTATACCTTTAAAATCAC205790.10134016321089602No Hit
AGACAGGACACCCTCCAGCTCTGGTTCTTGATGACCTCAGCAGCTTCCCC204810.10085756755538955No Hit
AGGCAGCAGAGGTAGAGCTTCCTTGTTGCCACAAATGAGAACACAAGAAT203380.10015337185398723No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)