FastQCFastQC Report
Thu 4 Jan 2024
G219M18_CKDL230043734-1A_22GGGVLT3_L8_R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG219M18_CKDL230043734-1A_22GGGVLT3_L8_R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18960963
Sequences flagged as poor quality0
Sequence length150
%GC53

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGGTGACCAGGCTCAAGGGCTGAACCAGAACAGGAGAGGAGACGCGAGG1586580.8367612974087866No Hit
AGGGTTTGAGAACTCCATTTCCCAGAGTGCTGTGCGGGTCGAGGGTGGTG936890.4941151986847926No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG850410.44850570089715375No Hit
GCCTAGGAGATGGTTGTCTTAGAGGAGTGAGTCTTATGGGGGTGGGAGGC780700.4117406905967803No Hit
TGGCTCAAACTATTGGCAATCCTTCTACTCAGTCTCTTGGGTGCTAGGAT576240.3039086147681423No Hit
ACGCTCACAAATACAAGAAGCCCTTTCGTGGGTAGGGAGAACAGGCTCCA574800.3031491596708458No Hit
AGCAACGTGTTCGAGGACTTCTACGAGGTGTCGCCGCTGCACATCAACTG556780.2936454229671774No Hit
CATGACAGCATCACAGGTAAGGAAGGAAGAGACTTCAGCCCCAGAAGAGA436800.23036804617993295No Hit
GGGAGGGGTTGCAAGGGCGAGCAGCTCATTGCAGGTTACTCCCAAGGAGC432870.22829536664356131No Hit
CCCCAATAGCCATAGACTGGGCCCATAGTAGGGATGCCGTCGAGGCGGGC424130.22368589612247014No Hit
TGGGTGGGGAAGCAGACGTTGGCAGGCCATGATGGACACCCGAGTGTGAA420580.22181362834788504No Hit
AACACTGGCAGGAAGGAAGCTGGGGATGCCAGCATCTAGAACACTCTTTG410300.2163919627921852No Hit
AGACTAAGTGGCTGCTACTCGCTTGTCCAGCACAGATAGCTTCTTTCCCA401070.211524066578264No Hit
CTCCTCGGCTCCCTCCTCCTTCCGACATGCAGCCAACACTCTCATGCCTC359010.18934164894472924No Hit
GGCAGGCGGGCGGGCGGGCAGTGTCCCTGAATTACTCAACAGAGCCATCT312010.16455387840796906No Hit
CTCCTGTGCCAAGCAGGGAGGGCACTGGGGCTCCAGCCAGCATCCTTCAA311520.16429545271513898No Hit
ACATTGAAGAACCGAGGTCTGATATAGTCCTTATACATAGAGTAGAGAAC291750.15386876710850603No Hit
TGAGAGTCAGCACACAGAGGCTATGGTCCAGGGGTGACCACGGTTCACTT285240.15043539718947818No Hit
GACCCAGCCACAGTCATCTGCCCTGAACCAAAATGACCGTGAAAGAAAGA282940.1492223786312963No Hit
ATGGAAAAGCTGCCAGAACAAAGGGGTCAGCTCCAGCAGCAGGCACAGGC275900.14550948704451352No Hit
GGGACAGGAGCTAAAGTCCAACTCTGTCAGCAAGCTGCTAGCCAGATGGG258590.13638020389576205No Hit
TCTGTGACCATTGGACCATTTCCCACCTTCCCCTGTTAAATACTTGGCTT252600.13322108165075794No Hit
GGTCGCAGCAAGCAGAGGACGAGCTGAGAAGGGAGCTTCCACTCTGGCCT251380.1325776544155484No Hit
TCTGGTGCTTTGCTACAAAGTTAAACATTCTATGGGCTGTGGTAAAGCAG240460.1268184532610501No Hit
CTGGTGCAGGCGGCAGCCCAGAGCAGCATGTCAATAGCCAGGCCAGACAC237610.1253153650476508No Hit
CCCTGGAGTCTGATTCCAGCTCCAGAGCTGCTCTCTTGCTGCGGGGGACT232100.1224093945017455No Hit
CCACTTCTCCAGTCCGAGAGTGCTCGTCCCTAGTCCCCAGGTCCTCTGCC229720.12115418399371383No Hit
AGTGAGTCACACTGTGTCCCACAGTATACCCTTTTGTCCACATATCTTCA227290.11987260351702601No Hit
GGAGCAAGAATCTAGCCCTGCATGAGGAGACACTCAAGCAGCTCCACAAG225710.11903931250749236No Hit
CCCACAGCTGAGTGTGCCTCCCAGTGAACCCTGAAGCTGGGCTGTCTCCA225420.11888636668928683No Hit
GGACAGAGGTTGCAGGGGTGGAACACAGATATGAGGGGATGGGGAGATGA215730.11377586676372925No Hit
GCGGTCTCTCTTCCCAGAGGCCCCGGCTCTGCTTACGGCTGGCCAGAAGC209480.11047962068171326No Hit
TACCCTGGCCTCACCATCTCGAAGGAACGTGTCAGCAAAGTAGAGGCAGC201280.1061549458221083No Hit
ATAGCTTCTTTCCCACCACCTGTCGTTCAGGAAGCCTGGTGCAGGCGGCA197420.10411918424185523No Hit
ACTTCATGGCTCCCACTGGGAATCCTGGGCTGGAATACTGACCCAAGTTC195180.1029378096460607No Hit
GGTCAGATAGAGACAGTATACACTCTTGTGACCCCATGAGAGAGGACCAA195110.10290089168994213No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)