FastQCFastQC Report
Tue 14 Feb 2023
G198M4_LC_R2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG198M4_LC_R2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences49
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAGCGAGACCGAGGCGGAGCGGGCGCCAGGCCCGGGGCGGCGACGCGGG24.081632653061225No Hit
CTGGTCAATCAATGTGAGTGCAGCAAATCAAACTGCTTGCAAGGTTAGAT24.081632653061225No Hit
TCCATTCAACTGCTTGTTACCTTGCGAATGTAGTTTTGGCAGGCATTTCA24.081632653061225No Hit
TCAGGCTCTGCCCTTGGAGTACCCAGCTTCCCTGCACATCCGGGAAGCGT24.081632653061225No Hit
GGTTATGAAATTGCCAAGTTTGCAAAGGGCAAGGAACAATTCTTGGCCTC24.081632653061225No Hit
AGAGCCAAGAATATCTCCATAGGAGCTAGAAAGGAAGCAGGACATCTCCA24.081632653061225No Hit
GCATCTAGAATAACAGCCGACCTTTATCTAACCTGCTGTGAACTGTGGCA24.081632653061225No Hit
GAGCTGCGGAGGGTGGCTGTGGCTGAAGTGATGGATGAGGCAGACGAGGC12.0408163265306123No Hit
GAGAGGAAGCAGAGGAGAGATGGGGAGCCTGGTCCCAGTAGCTGACCAGG12.0408163265306123No Hit
TGTCACCTTGCTCATCAGGCCTCCCTTATGCACTTTATGTGAACACACAC12.0408163265306123No Hit
TCACTCTGTAGACCAAGCTGAGCTTGAATTTAGAGATCCACCTGCCTCTG12.0408163265306123No Hit
GAAGAGGGATGCTTATTGTAAAGGATTTTTGCTATAGAGCATTCCTGGGG12.0408163265306123No Hit
AGAAGAAGCTCTGGCAGGCTCTGAGCTTCATCATACCAGGGGGGACCAGG12.0408163265306123No Hit
AACCTTGCCCAGGTCAGTCAGCAGCTGCTTGAGCTGCAGAAGCCTGGCCA12.0408163265306123No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAATCCAGGCAGTGCACATTAAACC12.0408163265306123No Hit
TCTGGGAGCCTCAGGGTATCTGGGTGAGGCCTGCATGAATCGGGGTTACT12.0408163265306123No Hit
AGGAGACTGGTATAGCCCTGTGAGGACAATATTCAGCTCTTAATATCCCC12.0408163265306123No Hit
TATATGAGCTGATGAAGGAGTCAGAATGCTAGAGAATCACGGTGTTCCAA12.0408163265306123No Hit
AGAGCCAGACGCCCCCCCCCCCCCCACCCACCCCAAAGAAAGTTGGGGGG12.0408163265306123No Hit
AACTGTGGGGCCATGATGGCATTGCCTTGAGCCTGAAGAGAACCTACGGA12.0408163265306123No Hit
CTTGCTGTGCTGAACTGGCTGGGACCGCTGAGGCTGCAGTTTTGGAGTGA12.0408163265306123No Hit
ATTAGAAGGGTGTCCACTAGGGGGCATGCCACGGGGGGGGGGGGGGGCGG12.0408163265306123No Hit
GTGTGCACACGCACACACAACTTACATAAAACACCTCTGCTATCCTATAG12.0408163265306123No Hit
GTGGCCACCCATAGAGACCTAGGACTTGTAGGTGATCAAAGCAGTAGAAA12.0408163265306123No Hit
TCCATTCAACTGCTTGTTACATTGCGAATGTAGTTTTGGCAGGCATTTCA12.0408163265306123No Hit
AGCGCATTGAGCGGGTGGACTGGCCCTGAGCCCAGTGAGCACCGGCCACC12.0408163265306123No Hit
GGGTGCTTTCTGTAAAGCACTATTAAAAACAAACAAAAAACCAAAAACAA12.0408163265306123No Hit
GGTTAGGAATGCCCATGCGTGCCTCAAGCCCCTTGGCAAAGGACAAGACC12.0408163265306123No Hit
ACCTTAGAGGGCACCAGGTCCACATGTGATACACATACATACATGTAGAC12.0408163265306123No Hit
ATTGTACCAAGTGAGTCACAAAGACACAAGTCGAAACAAAACACACCCAT12.0408163265306123No Hit
GCCCCACTCTGAAGGAACAATAAAACTATCTCACGCGAATCTAGAGCCGG12.0408163265306123No Hit
TCCCAAGTAGCCGCTGATGCCCGGCTACTCAACTTTAACCCAAAAGGAGG12.0408163265306123No Hit
AGAGCCAGACGCCCCCCCCCCCACACACCCCCCAAAGACAGATGGGGTTT12.0408163265306123No Hit
GAAGAGAGAGGCACACACACACACACACACACACACACACACACACACAC12.0408163265306123No Hit
CTAGCAAGGAGCTGGAGAACTGAGAACCCTGGAGGAAGTTCAGAGCGCTC12.0408163265306123No Hit
GCTACCTGGAAGCTGCCACGTCAGCAGTCCTCATGTCCTGAGAAACCCTA12.0408163265306123No Hit
ATGGCTCCCACTGGGAATCCTGGGCTGGAATACTGACCCAAGTTCAGTGC12.0408163265306123No Hit
ATCCAGCTGCCACTGGGTGAGCTGAGTGACCCTTCCCAGCTAGTAACTTA12.0408163265306123No Hit
ATGACCACTGAGAAAGATAAGGCTCTCCACACACGTGTTAGTGACTGTCA12.0408163265306123No Hit
TAGGGAGGGCACTGCCAATTGTGAAATCCCAATCTGCACTATGGATGGTG12.0408163265306123No Hit
TTCAGACCCCAGGACTACTCTGGTGAGCCAAATGGGCTTCAGGACATTTG12.0408163265306123No Hit
ATCCCTGTGAGTTCAAGGCCAGCTTCCTTTACATGGCAAGTTCCAGGACA12.0408163265306123No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)