FastQCFastQC Report
Tue 14 Feb 2023
G198M4_LC_R1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameG198M4_LC_R1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences49
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTGCAGCCTCTTCTGCTACTGCTTCTTTCCCAGACACCTCAGCCCCATT36.122448979591836No Hit
GGGTTAGTAGGGAATATTTGCCACCAGGAGCCACCAGAAGCATGTGACAA24.081632653061225No Hit
CTCTTCTCAGGCGGCTCCTGCCCTGAGCTGCTTAGAGGTGAGTCTGTTTC24.081632653061225No Hit
GCCTGCTGGCCTTCGCCGAGGATGCGCCCCCGTTCCGCCCGCGCCGTCGC24.081632653061225No Hit
CGTTGGTGCTGATAATGTCACTGGACAGAACCTTCGCCTTCTTGTTTATG24.081632653061225No Hit
CATTCCTGCCTGGCCACAGCAGGTAGCCTCTCCAATAGTCATTTCTTAAG24.081632653061225No Hit
TCCCCAGCCCCTTTGCTTGTCCTTATATACTCTCTGGGTTCAGCGTTTTT24.081632653061225No Hit
TGTTATTCTAGATGCCTTGGTCTCTTCTGCCTACTGTATTACCTTTCAGC24.081632653061225No Hit
TCCCATGACAGAGAATTTCTGCTATTGGCTTCCTGCAAACTGTTCCCGAG12.0408163265306123No Hit
GGTTTTTCACACCGACTCACCTTGAATGTCTCTGTGCTTTGCACTGGATT12.0408163265306123No Hit
ATGCTCTTAACCACGGAGCCATCTTTCCAGCCACGTATGTCTTATTGGCA12.0408163265306123No Hit
GAGTCCAACTTCTTTCCCAGGAACACTGTCTACAGCATAAGAGCGAGCTG12.0408163265306123No Hit
TGAGGTGGGTCTTGGGGGAAAGAAAGGATTTTATCCATGAAAGGATGGGG12.0408163265306123No Hit
TGGGGTCCCTTACTGTGCCAACAGCCACAGGCTGGTGCCAGGAATGCCAC12.0408163265306123No Hit
TAGCCACTGCACAGCAAATCTGAAACAGCTCTGCCTCCTGCTCTTCTGCC12.0408163265306123No Hit
CTTCTACAGCTTCTGCCGCCACTGGCAGCATCATTATGTTTATTTAGCCT12.0408163265306123No Hit
ATTAGTTTGCAATCTAGTCCCCCACTACCAAGCTGCTCCAGTGTACCAGA12.0408163265306123No Hit
GTATGTAGATGTTCTGTTCTAAATAATATGAAATAATACATATTCGTGTA12.0408163265306123No Hit
GGTGCCTCGGGTGCCAGGGTCGCATCGGGTGACTCGGGGAGTGTGTCTGC12.0408163265306123No Hit
TTGCCCTTTGGCCAGCCCCTTTTAAAAGTTCCACCACCTTCCAAAAGCAC12.0408163265306123No Hit
AACCACTGCCCTGGGCAGAGAGTCCAGCCAGGGGATCCGTGTGCCTGCCA12.0408163265306123No Hit
CATAAACAGTTACAAAATACTAAAAAAAAAAAATCAAAGGATAAACAAAA12.0408163265306123No Hit
AAGTTGCTTTGGTCATCATGTTTCATCATAGTAACAGGAACTCTAACTAA12.0408163265306123No Hit
TCCCTCCCACCCATACAAACCCCCCCCCCACCACAAAAAAAAAAAAAAGT12.0408163265306123No Hit
ACAGAAAAGCACTGCGTTTCACTCCCCGAAGAGGGTGCTGACACTAAGCT12.0408163265306123No Hit
ATTTATTCCCATTGAGAGTTAGCACATTTTAGTCATTGAGGCCATAACAC12.0408163265306123No Hit
AAAGAGGACCATCCTCTGAGGTGCCCACCTGCTCTCCAGTGCTGGGCTGG12.0408163265306123No Hit
TAAAAACAGTGACCTCTTATTAAAAGGTATGATTTCTTCTAAGTTCAGCA12.0408163265306123No Hit
CCTGGCACCTGGTGTTTCATTAGCCTTGGGCCTCGTGGAGGTTGGCGCCA12.0408163265306123No Hit
CTATTAAATACAGCATAAGGCAAATGGGGGCCTGTTAAAATCTCCCCAAC12.0408163265306123No Hit
TGTTATTTCATTGTGACCTTTGACAAGTTGCTTAACCTCTCTGAGCCTGT12.0408163265306123No Hit
TGAAATATCTTATTGAGACAATGTCTTGCTACGTAGCCTGGGTTGTCTTC12.0408163265306123No Hit
CAGACTTCTTCCCCAACTGCTTCCCCAGGTTTCCCTGGGGATTTGCAAAC12.0408163265306123No Hit
AGACAGGAGACAACGTGAACACTTAACAGGCAGTAATAAGGTGGGGGAGG12.0408163265306123No Hit
TGAAAAATAAAATATTTGACCTTTGTAACTTGATTTTTATGGTGAATGTG12.0408163265306123No Hit
GGGCCGGAGGGAGGGAGGGAAGGAGGGAGGGAAAGAGAGGAAGACTTCAG12.0408163265306123No Hit
TGACTTAGGAGGAAGGTCAGAAAAACATGGTGATTTCGGGCACAGAATCT12.0408163265306123No Hit
CCGCTGCCAACCGGGCAGGTGCACGAGCCCGCCGCAAGGCTGTCTGCGCC12.0408163265306123No Hit
TGGCAGTTCAACCTGCTCACACATGTGGAGCAGGATGAGGTGAGGAACCA12.0408163265306123No Hit
AAGCTCTACCCTTCATTGTTTTCTTCAGCTACAGTAAATGTGAGTCTCTC12.0408163265306123No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)