G124-G125-M20M38-NEBNext3_TTAGGC-_AC8KUAANXX_L008

Basic Statistics

Measure	Value
Filename	G124-G125-M20M38-NEBNext3_TTAGGC-_AC8KUAANXX_L008_001.R2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	16210328
Sequences flagged as poor quality	0
Sequence length	50
%GC	50

Per base quality graph

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG	329736	2.0341105991192774	Illumina Single End PCR Primer 1 (100% over 50bp)
CGGCGGGTGCCGGCGCGGGTCCCCTCCCCGCGGGGCCTCGCTCCACCCCC	105238	0.6492033967480485	No Hit
GGCGGGTGCCGGCGCGGGTCCCCTCCCCGCGGGGCCTCGCTCCACCCCCC	51655	0.3186548723751919	No Hit
CCGGCGGGTGCCGGCGCGGGTCCCCTCCCCGCGGGGCCTCGCTCCACCCC	44362	0.2736650362657684	No Hit
CCGCCCTCTCCCACGTCCGGGGAGACCCCCCGTCCTTTCCGCCCGGGCCC	43359	0.26747762290806204	No Hit
CTCCCACGTCCGGGGAGACCCCCCGTCCTTTCCGCCCGGGCCCGCCCTCC	26740	0.16495656349458196	No Hit
CCCACGTCCGGGGAGACCCCCCGTCCTTTCCGCCCGGGCCCGCCCTCCCC	21344	0.1316691432770515	No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN	21032	0.12974444440606014	No Hit
GCGGGTGCCGGCGCGGGTCCCCTCCCCGCGGGGCCTCGCTCCACCCCCCC	18976	0.11706117235875795	No Hit
CGGCGCGGGTCCCCTCCCCGCGGGGCCTCGCTCCACCCCCCCCATCGCCT	18773	0.115808884311286	No Hit
CGGTGGCGCACGCCTGTAGTCCCAGCTACTCGGGAGGCTGAGACAGGAGG	18403	0.11352638885530261	No Hit
CCCTCTCCCACGTCCGGGGAGACCCCCCGTCCTTTCCGCCCGGGCCCGCC	17717	0.1092945189017767	No Hit

Adapter graph

Kmer graph

Sequence	Count	Obs/Exp Max	Max Obs/Exp Position
GATCGGA	61500	43.442932	1
TCGTGTA	60640	43.409603	14
CGTCGTG	61145	43.408527	12
AGCGTCG	61155	43.33785	10
CGTGTAG	61095	43.2792	15
GAGCGTC	61370	43.17486	9
AGAGCGT	61555	43.145893	8
AAGAGCG	61485	43.02517	7
GATCTCG	45675	42.91827	34
ATCTCGG	46160	42.791725	35
ATCGGAA	62120	42.790054	2
GTCGTGT	62330	42.59525	13
CGGAAGA	62155	42.592495	4
TCGGAAG	62730	42.508274	3
GTAGGGA	62010	42.46253	18
TGTAGGG	62825	42.343033	17
GTCGCCG	40095	42.33995	44
GTAGATC	48380	42.153645	31
GAGTGTA	56845	42.07812	27
TGGTCGC	39455	42.06813	42